The first two editions of this title had a tremendous impact in neuroscience. Between the previous edition in 1989 and today, there has been an explosion of information in the field. These include advances in molecular techniques, such as genomics and proteomics, which have become increasing important in neuroscience. A renaissance in fluorescence has occurred, driven by the development of new probes, new microscopes, live imagers, and computer processing. The introduction of new markers has enormously stimulated the field, moving it from tissue culture to neurophysiology to functional MRI techniques. As with previous editions, this new edition will be welcomed by the graduate students working in the lab who needs fast, complete, up-to-date, and immediately applicable information about techniques, written by acknowledged experts in the field.