High-throughput sequencing and functional genomics technologies have given us a draft human genome sequence and have enabled large-scale genotyping and gene expression profiling of human populations. Databases containing large number of sequences, polymorphisms, and gene expression profiles of normal and diseased tissues in different clinical states are rapidly being generated for human and model organisms. Bioinformatics is thus rapidly growing in importance in the annotation of genomic sequences, in the understanding of the interplay between genes and proteins, in the analysis the genetic variability of species, etc. The 3rd APBC brings together researchers, professionals, and industrial practitioners for interaction and exchange of knowledge and ideas. The proceedings contains the latest results that address conceptual and practical issues of bioinformatics.Papers presented at APBC'05 and included in this proceedings volume span the following: Novel Applications in Bioinformatics, Computational Analysis of Biological Data, Data Mining & Statistical Modeling of Biological Data, Modeling and Simulation of Biological Processes, Visualization of Biological Processes and Data, Management, Migration, and Integration of Biological Databases, Access, Indexing, and Search in Biological Databases.