This thoroughly revised and updated third edition provides an overview of the principles and methodology of the most frequently used biochemical separation techniques for proteins used today. Balancing theory, procedures, and applications, it offers professionals and students in biochemistry, organic chemistry, and analytical chemistry quick access to a wide range of important techniques, including: Gel filtration and ion exchange chromatography Affinity and covalent chromatography  Reversed phase and hydrophobic interaction chromatography  Ligands from combinatorial libraries  Electrophoresis in gels  Immunoelectrophoresis and capillary electrophoresis  Two-dimensional electrophoresis in proteomics Protein elution and blotting techniques Large-scale electrophoresis processes

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